1. Nuclear fast red
2. 1% Bismark Brown in 1% acetic acid (gives pale,golden brown nuclei)
3. Basic fuchsin in 1% acetic acid, pinkish-red nuclei.
4. Scarba red.
5. Ask Vector for a recommendation.
Geoff
Melissa Mazan wrote:
> Hi all, I am doing phospho ERK staining - using the Vector MOM kit on
> lung tissue - I want to counterstain so that I can enumerate number of
> ERK pos nuclei with respect to other nuclei, but if I use hematoxylin
> it obscures the signal - everything just looks dark. Any suggestions
> for a nuclear counterstain? Many thanks - Melissa
>
> histonet-request@lists.utsouthwestern.edu wrote:
>>
>>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff@umdnj.edu
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