Re: [Histonet] DAB restain

From:Rene J Buesa

Bleach will definitely eliminate DAB, and antigen activity as well!
  René J.

MKing  wrote:
  I've lightened DAB immersing slides with dilute household bleach (sodium 
hypochlorite), to the point where it was no longer visible. Try 
1:20-1:100 dilution of the 5% commercial liquid. Don't know what 
happens to it next time you react tissue for DAB, would recommend 
another substrate or fluorescence, or at least appropriate controls.
Mike King

Message: 14
Date: Tue, 16 Oct 2007 13:58:04 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] crazy immuno question - can you restain
To: Cheryl Cross , histonet

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If the section already has DAB (as it probably does), you cannot 
eliminate it.
René J.

Cheryl Cross wrote:
hi everybody -

I'm going back through some of my earlier attempts at immuno for
cleaved caspase 3; i have a beautiful lesion i ruined :) on
dilutions. is there anyway i can use my newer, better protocol on
this previously stained slide?

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