What kind of sections are you using? Are they thick enough? Thin sections would show nuclei and not "branches" since "branches" would randomly be out of the plane of a too thin section. Mainly used flourescent tagging of GFP cells in mouse brain with anti-GFP and a flourescent secondary. But certainly have done this nicely with appropriate secondary and then DAB. Used a goat anti-GFP, (you can use others), a biotinylated horse anti-goat from Vector (I personally like Vector secondaries but can use others), avidin/Peroxidase and DAB. You can actually see a beautiful picture on AbReviews of mouse brain stained with GFP and DAB that I have to admit outdoes anything I did yet they have a very similar procedure although I certainly didn't write the review or produce the slide but mine were pretty darn good.
-------------- Original message --------------
> Dear everyone.
> Have someone ever tried to perform a DAB
> immunohistochesmitry in tissue transfected with GFP? Some people in my
> lab have been trying it with not very good results. This is
> hippocampus and apparently just the nuclei and not the branches are
> Histonet mailing list
Histonet mailing list