I used CD-3,T antibody from DAKO (polyclonal rabbit IgG) at 1:200 dilution, HIER at pH6 incubated for 30 minutes at RT and standard detection systems. Tonsil as control.
  René J.

Dahui You  wrote:
  Hello, everyone:
We are trying to identify CD3 positive T cells and/or H1N1 influenza in some HistoChoice (Amresco) fixed and paraffin embedded mouse lung sections.
Does anybody have experience with these antibodies? Any suggestions on companies and antibodies?
Thanks in advance and I appreciate your help.
Dahui

Dahui You
Department of Biological Sciences
Louisiana State University
Baton Rouge, LA 70803
Tel:225-578-5960
Fax:225-578-2594



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