Hi Sally
I will give you a call.
Best Regards
Mari Ann Mailhiot BA HT ASCP
Application Specialist
Leica Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot@leica-microsystems.com
www.leica-microsystems.com
"Breeden, Sara"
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[Histonet] AutoStainer XL Tweaking
10/16/2007 07:04
AM
I'm setting up my Leica AutoStainer XL and would appreciate any help
with tweaking the times. I based my timing for the Autostainer on my
manual stain times but staining is not crisp and looks washed out. Docs
say eosin is fine but cellular detail is foggy. Here are my times:
XYL - 3 at 5 min each
100% reagent - 2 at 30 seconds each
95% reagent - 1 at 30 seconds
80% reagent - 1 at 30 seconds
DI water wash - 1 minute
Hematox (currently using Sigma) - 5 minutes
DI water wash - 1 minute
Acid Alcohol (1%) - one dip
DI water wash - 1 minute
Ammonia water - 15 seconds
DI water wash - 1 minute
80% reagent - 1 at 30 seconds
95% reagent - 1 at 30 seconds
Eosin (alcoholic) - 10 seconds
95% reagent - 1 at 30 seconds
100% reagent - 2 at 1 minute each
XYL - 3 at 1 minute each
I cut my acid alcohol time down from 15 seconds to one dip but it's
still taking a lot of hematoxylin off. Should I try 0.5% acid alcohol?
I'm at a loss so far; different hematox? More time in ammonia water? If
you use this Leica AutoStainer XL, could you offer some Handy Hints? I
thank you SO much!
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
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