Yes, soaking the faced block is a cure for poor processing, but it works. I
find that holding a moist ice cube against the block face for about 30
seconds does wonders for a poorly processed block.
Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida 33161
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo
Rogerson
Sent: Friday, October 26, 2007 7:24 AM
To: Weems, Joyce; Kaliko, Bonnie; Mike Pence; Cheri Miller; Smith Wanda;
Anna K. Schultz; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] curious about soaking paraffin blocks.
"The key to the perfect section after soaking is not to leave the block
so long that the tissue is damaged. The block then has to be faced to
the exact spot that the perfect section can be taken - not where the
tissue is still too soaked or after you've moved past that place again.
Once again this part has to do with perception and experience."
Helpful.... so obviously a way of answering poor fixation and/ or
processing; sorry but I never came across this in the UK. Do any of you
Brits do this?
Kemlo Rogerson
Pathology Manager
DD 01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
Biology is the least of what makes someone a mother. --Oprah Winfrey
This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on
its contents: to do so is strictly prohibited and may be unlawful.
Please inform me that this message has gone astray before deleting it.
Thank you for your co-operation
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses.
Barry University - Miami Shores, FL (http://www.barry.edu)
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
|