If you have really old slides (like the glue is drying out and yellowish)
you can spray them with histofreeze, slip the edge of a scalpel under the
edge of the coverslip and pop it off. Just wipe the condensate form the
histofreeze off before you go into xylene to remove the excess mounting
media. I spray both sides.
Bernice

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Friday, October 19, 2007 8:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] removing a coverslip

rant below:
Can I also note that if you coverslipped your slides aqueously, you CANNOT
remove the coverslips with xylene.
Our newest lab tech tried that.  Her reasoning was that the sections were
paraffin, though she had de-paraffinized them in xylene-EtOH-water steps and
kept them aqueous.
Explaining why she can't use xylene didn't help.  The next time she says she
has eight years of experience, I think I'll bring this mishap up.
Sigh.

Emily
-- 
Yog-Sothoth knows the gate.
Yog-Sothoth is the gate.
Yog-Sothoth is the key and guardian of the gate. Past, present, future, all
are one in Yog-Sothoth.
He knows where the Old Ones broke through of old, and where They shall break
through again.
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