"I don't agree.
If the perfusion pressure does not match physiological pressure at
least, you will not wash out all the blood. Fixative will reach some
places much later than others. Red blood cells that autoflouresce and
catalyze HRP reactions will remain behind.
To match physiological pressure, you would need about 3 meters between
the subject and the fluid reservoir. Do the math.
I use double phyisological pressure, and get excellent perfusions free
of red blood cells and with no damage. EM and Nissl sections look
Anyway, you just need a longer tube and longer arms then! The logic that
you don't need a pump still stands.
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