RE: [Histonet] frozen sections

From:"Weems, Joyce"



I never used it for fluorescence, so I don't know! Sounds like that might be a problem for you. I used it for muscles and would freeze them on the chuck. I rolled the tissue in the powder and thickened the OCT with it. They stood on end for freezing and I was never aware of any problem caused by the talc. 

Joyce Weems
Pathology Manager
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax


-----Original Message-----
From: Sharon Bledsoe [mailto:sbledsoe@iupui.edu]
Sent: Wednesday, October 17, 2007 8:33 AM
To: Weems, Joyce; Atoska Gentry; Histonet
Subject: RE: [Histonet] frozen sections


Joyce,


How does the baby powder effect your sample if you have to use 
birefringence?  Talc polarizes, I have to be careful and use 
non-powdered gloves when I section many of our animal tissue as we 
are looking for crystals that are identified by birefringence.

Do you note on the slide that you have an additive  on a frozen section?



Sharon Bledsoe










At 3:58 PM -0400 10/16/07, Weems, Joyce wrote:
>Content-class: urn:content-classes:message
>Content-Type: text/plain;
>   charset="utf-8"
>
>Please the tissue in baby powder and it will eliminate the air 
>bubble. You can also add baby powder to the OCT and it will give it 
>more "body" for holding the tissue correctly for freezing. I don't 
>remember where I heard this or learned it... I just know it works! 
>Hope it works for you, j
>
>Joyce Weems
>Pathology Manager
>Saint Joseph's Hospital
>5665 Peachtree Dunwoody Rd NE
>Atlanta, GA 30342
>404-851-7376 - Phone
>404-851-7831 - Fax
>
>
>-----Original Message-----
>From: histonet-bounces@lists.utsouthwestern.edu
>[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Atoska
>Gentry
>Sent: Tuesday, October 16, 2007 3:55 PM
>To: Histonet
>Subject: [Histonet] frozen sections
>
>
>hello, has anyone observed empty space surrounding tissue frozen in
>O.C.T. or similar tissue freezing medium after snap freezing? Upon
>sectioning samples shipped previously snap frozen to one of our
>researchers I noticed space surrounding various areas of the tissue.
>Does anyone know what probably caused this? Could this be a factor in
>the generation of horrible sections? I suspect the tissue may be moving
>when the blade touches it. Is there a know remedy that will not damage
>the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any
>assistance will be much appreciated. Atoska
>
>--
>Atoska S. Gentry, B.S., HT(ASCP)
>Research Assistant IV
>Scott-Ritchey RSCH Center
>College of Vet. Med
>Auburn, AL 36849
>PH (334) 844-5579
>FAX (334) 844-5850
>email: gentras@vetmed.auburn.edu
>
>
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Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc.




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