RE: [Histonet] curious about soaking paraffin blocks.

From:"Kaliko, Bonnie"

Kemlo, you obviously do not work with animal tissue which is VERY dry.
It has nothing to do with poor fixation or over dehydration. Liver,
bone, spleen, muscle is impossible to cut unless it is placed face down
on ice.  If you ever try cutting animal tissue and manage to get a good
section without soaking it, then you should apply for a patent

-----Original Message-----
From: Kemlo Rogerson [] 
Sent: Friday, October 26, 2007 7:24 AM
To: Weems, Joyce; Kaliko, Bonnie {Nonc~Nutley}; Mike Pence; Cheri
Miller; Smith Wanda; Anna K. Schultz;
Subject: RE: [Histonet] curious about soaking paraffin blocks.

"The key to the perfect section after soaking is not to leave the block
so long that the tissue is damaged. The block then has to be faced to
the exact spot that the perfect section can be taken - not where the
tissue is still too soaked or after you've moved past that place again.
Once again this part has to do with perception and experience."

Helpful.... so obviously a way of answering poor fixation and/ or
processing; sorry but I never came across this in the UK. Do any of you
Brits do this?

Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
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