I believe overdehydration has something to do with extracting the bound
I rehydrate - have for years, since we are trying to rush processing
without the adequate fixation we used to have by leaving large specimens
overnight in formalin. I blow on the blocks, put some of the colon bxs
in the water bath for a few seconds, put large specimens on ice -
anything to get a good section.
The key to the perfect section after soaking is not to leave the block
so long that the tissue is damaged. The block then has to be faced to
the exact spot that the perfect section can be taken - not where the
tissue is still too soaked or after you've moved past that place again.
Once again this part has to do with perception and experience.
My 2 cents again...j:>)
Saint Joseph's Hospital of Atlanta
404-851-7831 - Fax
[mailto:firstname.lastname@example.org] On Behalf Of Kemlo
Sent: Friday, October 26, 2007 2:54 AM
To: Kaliko, Bonnie; Mike Pence; Cheri Miller; Smith Wanda; Anna K.
Subject: RE: [Histonet] curious about soaking paraffin blocks.
"Well as far as animal tissue is concerned, ice and water are needed to
hydrate the tissue in order to obtain a section. Each tissue responds
differently, some needing time on the ice longer then others."
That statement makes no sense, you rehydrate after sectioning and drying
off and prior to staining with aqueous stains; you don't rehydrate prior
I can't believe you believe what you appear to have said.
DD 01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc.
Histonet mailing list