Well as far as animal tissue is concerned, ice and water are needed to
hydrate the tissue in order to obtain a section.  Each tissue responds
differently, some needing time on the ice longer then others.  


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo
Rogerson
Sent: Thursday, October 25, 2007 10:41 AM
To: Mike Pence; Cheri Miller; Smith Wanda; Anna K. Schultz;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] curious about soaking paraffin blocks.

"So if you place the block on the ice tissue up, what are you soaking,
paraffin?"

Well if soak it in ice tissue side down what are you cutting frozen
sections? Don't understand why you'd want to potentially stick water
back into something you've dehydrated; makes no sense. Why not just put
blocks face down on a chiller or one of those Peltier effect thingies. 


Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
 
Biology is the least of what makes someone a mother. --Oprah Winfrey 
This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on
its contents: to do so is strictly prohibited and may be unlawful.
Please inform me that this message has gone astray before deleting it.
Thank you for your co-operation
 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet