RE: [Histonet] baby powder freeze aid

From:"Hofecker, Jennifer L"



I believe you are correct. We use talc (purchased from Fisher) to freeze
our muscle biopsies.  We have received some samples from outside
institutions that were obviously frozen with baby powder (smell powder
fresh when you open the package). The samples tend to have some "ice
artifact" which is the reason we encourage the use of talc in the first
place.
If you are looking to reduce freeze artifact (and you are not using
fluorescence) go ahead and order the talc from a Scientific product
distributor. It's relatively inexpensive.


Anybody know if talc protects from "flaming"????

Have a great rest of the week.

Jennifer L. Hofecker, HT(ASCP)
Vanderbilt University Medical Center
Division of Neuropathology 
Nashville, TN
ph. (615)343-0083
fax. (615)343-7089
NSH Quality Control Committee Chair
 
 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MKing
Sent: Thursday, October 18, 2007 11:18 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] baby powder freeze aid


Isn't baby powder talc-free these days, cornstarch?

Message: 24
Date: Wed, 17 Oct 2007 08:32:49 -0400
From: Sharon Bledsoe 
Subject: RE: [Histonet] frozen sections
To: "Weems, Joyce" ,	"Atoska Gentry"
	,	"Histonet"

Message-ID: 
Content-Type: text/plain; charset="us-ascii" ; format="flowed" Joyce,
How does the baby powder effect your sample if you have to use
birefringence?  Talc polarizes,...

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