Has your fixation or processing times been altered? The fact that the
control section adheres and the patient tissue does not, makes me think
that you might have a problem with fixation (since you even tried drying
overnight). You might want to check it out.
Mark Adam Tarango HT(ASCP)
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV 89135
Direct Line (702) 822-5112
Treo (702) 759-9229
Fax (702) 939-7663
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kate
Mendell
Sent: Tuesday, October 23, 2007 11:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Help Tissue falling off
We have been having a problem with tissue falling off our slides during
IHC (benchmark). Nothing has changed we are using the same protocol,
same slides, and same control. The controls are staying on and we don't
double dip. It doesn't matter if we air dry overnight, microwave or oven
dry not all antibodies mostly CD's. Anyone else having problem. We use
Colorfrost Plus Micro Slides from Erie Scientfic bought through Cardinal
Health.
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