RE: [Histonet] Help Tissue falling off

From:"Kari Bradshaw"



Most recently there has been a problem with a certain lot of detection.
If you have another detection kit, I would suggest putting it on.  Make
sure to call Ventana and let them know if it is indeed the detection
kit.

Kari Bradshaw
Anatomic Pathology Manager
Lower Columbia Pathologists
1217 14th Ave
Longview, WA 98632
360.425.5620
kbradshaw@lcpath.com
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kate
Mendell
Sent: Tuesday, October 23, 2007 11:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Help Tissue falling off

We have been having a problem with tissue falling off our slides during
IHC (benchmark).  Nothing has changed we are using the same protocol,
same slides, and same control.  The controls are staying on and we don't
double dip. It doesn't matter if we air dry overnight, microwave or oven
dry not all antibodies mostly CD's.  Anyone else having problem.  We use
Colorfrost Plus Micro Slides from Erie Scientfic bought through Cardinal
Health.

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