We are having a problem with our H&E stain. The pathologist is seeing
circular areas on the tissues that are not staining with
the hematoxylin(Harris), but they are picking up the Eosin. We
are changing the first xylenes and alcohols and hoping that might help.
Any idea why this might be happening? Thanks!
Old chestnut this one; answers usually are poor dewaxing, sections
lifting and/ or poor fixation.
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