RE: [Histonet] H&E problem

From:"Weems, Joyce"

I'd be sure the slides are completely deparaffinized.

Joyce Weems
Pathology Manager
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax

-----Original Message-----
[]On Behalf Of
Margiotta, Michele
Sent: Wednesday, October 17, 2007 11:07 AM
Subject: [Histonet] H&E problem

Hi All,

We are having a problem with our H&E stain.  The pathologist is seeing circular areas on the tissues that are not staining with
	the hematoxylin(Harris), but they are picking up the Eosin.  We are changing the first xylenes and alcohols and hoping that might help. Any idea why this might be happening?  Thanks!  

Michele Margiotta
Histology Supervisor

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