RE: [Histonet] H&E problem

From:"Weems, Joyce"



I'd be sure the slides are completely deparaffinized.

Joyce Weems
Pathology Manager
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
Margiotta, Michele
Sent: Wednesday, October 17, 2007 11:07 AM
To: histonet@pathology.swmed.edu
Subject: [Histonet] H&E problem


Hi All,

We are having a problem with our H&E stain.  The pathologist is seeing circular areas on the tissues that are not staining with
	the hematoxylin(Harris), but they are picking up the Eosin.  We are changing the first xylenes and alcohols and hoping that might help. Any idea why this might be happening?  Thanks!  

Michele Margiotta
BMHMC
Histology Supervisor
631-654-7192




This e-mail and any files transmitted with it are confidential and are intended 
solely for the use of the individual or entity to which they are addressed. 
This communication  may contain material protected by the attorney-client 
privilege. If you are not the intended recipient or the person responsible for
delivering the e-mail to the intended recipient, be advised that you have received 
this e-mail in error and that any use, dissemination, forwarding, printing, 
or copying of this e-mail is strictly prohibited. If you have received this e-mail in 
error, please immediately notify the sender via return e-mail or call
Brookhaven Memorial Hospital Medical Center at (631) 654-7282.



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc.




<< Previous Message | Next Message >>