[Histonet] wax cracks along edge of biopsies

From:Patricia Valente



 Any suggestions about the cause of blocks cracking along the edge of biopsies (happens with some of our prostate biopsies about 14-20 mm long)We have tried to eliminate following factors    the temp of the cold plate (-5 C)    Care in removing blocks from molds    biopsies getting colder before  embedding- therefore wax not evenly impregnatedSeems to happen along one side of the biopsy causing splitting and or creases when sectioningsometimes only  a couple of blocks effected sometimes an irritating amount. usually solved when re-embeddedWhat technique can we change to eliminate problemother details- processing looks OK, cores are not hard, wax in use is polyfinThanks 

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