Any suggestions about the cause of blocks cracking along the edge of biopsies (happens with some of our prostate biopsies about 14-20 mm long)We have tried to eliminate following factors the temp of the cold plate (-5 C) Care in removing blocks from molds biopsies getting colder before embedding- therefore wax not evenly impregnatedSeems to happen along one side of the biopsy causing splitting and or creases when sectioningsometimes only a couple of blocks effected sometimes an irritating amount. usually solved when re-embeddedWhat technique can we change to eliminate problemother details- processing looks OK, cores are not hard, wax in use is polyfinThanks
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
|