hello, has anyone observed empty space surrounding tissue frozen in
O.C.T. or similar tissue freezing medium after snap freezing? Upon
sectioning samples shipped previously snap frozen to one of our
researchers I noticed space surrounding various areas of the tissue.
Does anyone know what probably caused this? Could this be a factor in
the generation of horrible sections? I suspect the tissue may be moving
when the blade touches it. Is there a know remedy that will not damage
the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any
assistance will be much appreciated. Atoska
--
Atoska S. Gentry, B.S., HT(ASCP)
Research Assistant IV
Scott-Ritchey RSCH Center
College of Vet. Med
Auburn, AL 36849
PH (334) 844-5579
FAX (334) 844-5850
email: gentras@vetmed.auburn.edu
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