Did you happen to use a tissue adhesive in your waterbath? The sticky
adhesives often hold on to the IHC reagents and show up as a film around the
Good luck troubleshooting,
Date: Thu, 25 Oct 2007 14:02:50 -0400
From: "Diana McCaig"
Subject: [Histonet] background staining after DAB
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We are using the Biocare IHC protocol and using Fisher superfrost plus
slides (due to decloaking). When doing the double stain, there is a lot
of background stain from the DAB. We do wash well so that is not the
problem. I am wondering if is the charge on the slides. I have checked
the pH of all the solutions and they are okay. Any ideas. The stain is
readable, but does not look too nice.
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