[Histonet] Tris buffer

From:"Amos Brooks"



Sheila,
     The idea is to make sure the environment the sections are in before
adding the antibodies is as similar to the environment the antibodies
themselves are in as possible. This increases the avidity and affinity of
the antibodies for the antigens.
     Think of it like a lock and key. If your hand is shaking you won't be
able to get the key into the lock as well. The molecules of the proteins are
doing the same thing when they are of a different pH (or temperature for
that matter). When the molecules aren't moving around or twisting (like what
happens in antigen retrieval) they can line up and the reactive parts can
get close enough to bond properly.

Amos Brooks
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




<< Previous Message | Next Message >>