I have recently begun trying to optimise Timms copper stain on kidney
tissue. I have frozen tissue sections and I leave them in 1% sodium sulphide
for 1hr before TCA treatment and then the Timms reagent.
The recipe I am using is:
5% silver nitrate aqueous (5g in 100ml distilled H2O).
Citric acid (monohydrate) 5.0gms
Distilled water 100ml
Develop sections in freshly filtered solution of 1 part A and 5 parts B for
There are a number of different recipes for the Timms reagent and the one I
am currently using is not working. I cannot seem to get the stain into the
tissue. I end up with lots of staining on top of the tissue despite heavy
Does anyone have a different recipe that I could try? I have seen recipes
for Timms reagent using gum arabic and 10% hydroquinone, except hydroquinone
is only soluble in water to 7% (I tried making a 10% solution and it was a
Any suggestions would be greatly appreciated.
Proteomics and Biomedicine Group
School of Biological Sciences
University of Auckland
Tel: (09) 373-7599 ext 85763
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