[Histonet] DAB restain


I've lightened DAB immersing slides with dilute household bleach (sodium 
hypochlorite), to the point where it was no longer visible.  Try 
1:20-1:100 dilution of the 5% commercial liquid.  Don't know what 
happens to it next time you react tissue for DAB, would recommend 
another substrate or fluorescence, or at least appropriate controls.
Mike King

Message: 14
Date: Tue, 16 Oct 2007 13:58:04 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] crazy immuno question - can you restain
To: Cheryl Cross ,	histonet
Message-ID: <474958.73016.qm@web61213.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

If the section already has DAB (as it probably does), you cannot 
eliminate it.
   René J.

Cheryl Cross  wrote:
   hi everybody -

I'm going back through some of my earlier attempts at immuno for
cleaved caspase 3; i have a beautiful lesion i ruined  :)  on
dilutions. is there anyway i can use my newer, better protocol on
this previously stained slide?

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