We use mayers hematoxylin 5 min as counterstain. - only nuclei-stain is
perhaps easier to distinguish from the fungi.
Gudrun Lang
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Ursprüngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Cheri
Miller
Gesendet: Donnerstag, 18. Oktober 2007 20:25
An: 'Histonet'
Betreff: [Histonet] FW: PAS/Fungus
Cheri Miller HT ASCP
Histology Supervisor
Physicians Laboratory Services, Inc.
Omaha, NE 68117
402 738 5052
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From: Cheri Miller [mailto:cmiller@physlab.com]
Sent: Wednesday, October 17, 2007 10:38 AM
To: histonet-bounces@lists.utsouthwestern.edu
Subject: PAS/Fungus
I have a Path that says our PAS/F is weak. His suggestion is that I do a
hematoxilyn counter instead of light green. Anyone else doing this?? Seems
to me that the counter stain won't make the PAS reaction any stronger. Our
procedure is as follows;
.5%Periodic acid 5 mins
2 changes DI water
Schiff's 20 mins
Running water 10 mins
Light green 1 min
95,100,100 and 3 xylene
I don't get any complaints from the other 6 pathologist's. Share your
wisdom. Thanks Cheri
Cheri Miller HT ASCP
Histology Supervisor
Physicians Laboratory Services, Inc.
Omaha, NE 68117
402 738 5052
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you are not the addressee intended / indicated or agent responsible for
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delete this email from your system.
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