Re: [Histonet] which mounting media for DAB + toluidine blue staining?

From:Rene J Buesa



Moran:
Once the DAB has reacted (oxidized) it is permanent, no matter which mounting medium you use.
René J.
Moran Elishmereni  wrote:  Hi histonetters,

I am staining skin sections for mast cells (toluidine blue) and for an
eosinophil protein (using DAB). Can I use entelan as mounting media? It
works nicely for the tol blue, but I fear it will ruin the DAB staining. Is
there any alternative?

Thanks,
Moran

-- 
Moran Elishmereni

Department of Pharmacology and Experimental Therapeutics
School of Pharmacy, Faculty of Medicine
The Hebrew University of Jerusalem
POB 12065
Jerusalem 91120, ISRAEL
Tel: 972-2-675-8746
Fax: 972-2-675-8144
Email: moran.elish@gmail.com
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