Re: [Histonet] stain living cells - what dye doesn't kill the cells?

From:Rene J Buesa

  Your question belongs to the realm of "supravital" or "vital" stains, i.e. those able to accomplish exactly what you want to do.
  I always used neutral red in dilutions between 1:10,000 and 1:50,000 depending on the subject . The idea being to let the living cells to incorporate and concentrate the dye in a way that they survive and become visible. 
  Now a question: why you don't use a phase or an interference optical system to view the cells, alive and without staining them?
  René J.

Moran Elishmereni  wrote:
  Dear Histonetters,

Does anyone know of a dye/stain (fluorescent or non-fluorescent) that does
not kill cells? I want to do some live-imaging of live primary mast cells,
but be able to distinguish them from other cells in a certain mix. So I need
to stain them- but not affect there viability/function.
I know that this isnt exactly the forum for this question, but I'm hoping to
get some suggestions anyway.
Many thanks,

Moran Elishmereni

Department of Pharmacology and Experimental Therapeutics
School of Pharmacy, Faculty of Medicine
The Hebrew University of Jerusalem
POB 12065
Jerusalem 91120, ISRAEL
Tel: 972-2-675-8746
Fax: 972-2-675-8144
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