Re: [Histonet] can I leave IHC sections overnight after first staining?

From:Rene J Buesa



If you are FORCED to leave them over night, leave  them in PBS.
  René J.

Moran Elishmereni  wrote:
  Hi Histonetters,

Is it possible to leave sections (that were stained with primary Ab)
overnight, and counterstain the day after? I am using DAB reaction. Can I
leave it dry (or wet, in distilled water) overnight?

Thanks,
Moran

-- 
Moran Elishmereni

Department of Pharmacology and Experimental Therapeutics
School of Pharmacy, Faculty of Medicine
The Hebrew University of Jerusalem
POB 12065
Jerusalem 91120, ISRAEL
Tel: 972-2-675-8746
Fax: 972-2-675-8144
Email: moran.elish@gmail.com
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