Run your control slides in parallel, one set on the instrument and one off 
using a manual method.  Compare the results.  This would rule out/in a 
kit/instrument problem.


Jennifer MacDonald
Director, Histotechnician Training Program
Mt. San Antonio College
1100 N. Grand Ave.
Walnut, CA 91789
(909) 594-5611 ext. 4884
jmacdonald@mtsac.edu



Schaundra Walton  
Sent by: histonet-bounces@lists.utsouthwestern.edu
10/10/2007 09:03 AM

To
Histonet 
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Subject
[Histonet] Iron Staining of Bone Marrows






We use a Vetana NEXUS special stainer and stain our bone marrow aspirates 
and smears with Ventana's iron kit.  One of our pathologists has 
complained (repeatedly) that our irons aren't staining like he expects. 
Has anyone else had trouble with Ventana's iron kit?  Is anyone using a 
different protocol?  Any advise would be greatly appreciated.

Schaundra Walton BS HTL(ASCP)
Swedish American Hospital
1401 E. State St. 
Rockford, IL 61104
 
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