Re: [Histonet] IHC and Cytology (IHC Forum)

From:"Richard Cartun"

I agree that cell blocks are most helpful, but, the simple truth is,
"they are not always available".  We do immunocytochemistry on cytology
smears, Thin-Preps, and Paps almost every day and we get very good
results with most antibodies.  I have performed validations for most of
our antibodies so I know which ones work on alcohol-fixed cells and
which ones do not.  I run paraffin sections for positive controls
because it's not practical to prepare cytology smears for this purpose
and I hardly ever run a negative control.  I don't think a negative
control is that important especially when you are using polymer
detection.  When possible, it is important to identify "internal"
positive and negative controls within the test slide.  We also use
heat-induced antigen retrieval for many antibodies because it is
essential even though the cells are not fixed in formalin.


Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Lott, Robert"  10/01/07 1:23 PM
I just had to weigh in on this...


Most of you are familiar with Dr. Rodney Miller.  He is the Director of
Immunohistochemistry at ProPath Labs in Dallas.

He is world renown for his expertise. He is also a board certified
cytopathologist and a true friend to NSH having spoken at many local,
state, and national meetings.


Dr. Miller wrote a book :-) called Practical Cytopathology.

The COMPLETE contents of this textbook are as follows:


Get a good cell block and do immunostains if you can't figure it out on
the H&E.



For more information follow this link: 





Robert L. Lott, HTL(ASCP)

Manager, Anatomic Pathology

Trinity Medical Center / LabFirst

800 Montclair Road

Birmingham, AL  35213


205-592-5646 - fax 



Date: Sun, 30 Sep 2007 11:03:18 -0700 (PDT)

From: Rene J Buesa 

Subject: Re: [Histonet] IHC Forum

To: patsy ruegg , 

Message-ID: <>

Content-Type: text/plain; charset=iso-8859-1


Hi Patsy:


This week there was a discussion in Histonet about IHC on PAP smears
and cytospins and using tissue sections as positive controls or not.


  Roughly the positions were the following:


  1- some accepted using FFPE tissue sections as (+) controls, after

  2- some suggested fixing the smears with NBF and cause the
"crossslinkage" to do HIER afterwards

  3- some advocated using ONLY known (+) smears or frozen sections as
(+) controls, i.e. non-fixed tissues. The rational being a CAP
instruction in which they direct to use controls treated the same as the


  I think this is an important topic but I also think the "discussion"
should be beyond an intelectual argument of each, because that will just
expand what has been said this last week.

  I think that with some time ahead some "hard evidence" ought to be
presented for discussion, i.e. results obtained using the 3 "approaches=22
summarized above.


  The panel then should view the slides, grade them and get to a
determination. The slides should be reviewed/graded "blind", i.e. no one
shoudl know the procedure s/he is evaluating.


  Give consideration to this proposal.

  René J.


patsy ruegg  wrote:



This is a call for IHC questions to be discussed at the NSH S/C in
Denver in OCT.




If you have any burning questions you would like our panel of 9 to
address please email them to me ahead of time.




Thank you,




Patsy Ruegg


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