RE: [Histonet] the ongoing saga of muCD31 staining

From:"Tarango, Mark"

You can use it on paraffin-embedded tissue.  Pyronin-Y stains RNA pink.
Lots of people use this with the methyl-green pyronin-Y stain.  It's to
show DNA and RNA in two different colors.  Methyl green stains DNA
greenish-blue and pyronin-Y stains RNA pink.  We sometimes use this when
looking for plasma cells, if the doc can't wait for a CD138 immunostain.
I've never used it as a counterstain for IHC.  

Mark Adam Tarango HT(ASCP)
Histology & IHC Supervisor
Nevada Cancer Institute
One Breakthrough Way
Las Vegas, NV  89135
Direct Line (702) 822-5112
Mobile (702) 759-9229
Fax (702) 939-7663

-----Original Message-----
[] On Behalf Of
Christian Franci
Sent: Wednesday, October 03, 2007 9:02 AM
Subject: [Histonet] the ongoing saga of muCD31 staining

hey you zany histokids!
All told, Iv'e gotten several Ab's to work on FFPE sections.  The 
staining is reproducible, clean but it's never super strong.  It seems 
that any time you have to do enzyme digestion there's a VERY FINE line 
between under-digestion and over-digestion of the section and when you 
have several dozen to do at once, well, the timing is not always 
anyhow, I found a nice paper in Nature from 2006 from Gavin Thurston's 
group over at Regeneron.  They have these beautiful images of mouse 
blood vessels.  Of course, being a Nature paper, the details are rather 
vague... but, I noticed something interesting...
they used pyronin-y as a counter-stain not hematox.  I've personally 
never used this or heard of it...
Have any of you used this stain? is it simple to use or are there 
"tricks" involved? Can it be used in FFPE sections or just in frozen 

As usual, thanks in advance for any help you can give me.

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