We have been using an in house cocktail of P63, high molecular weight
cytokeratin and AMACR (P504S) for some time now with good results on
prostate specimens. All antibodies obtained from DAKO.
P63/34BE12 with Polymer peroxidase and DAB. 
P504S with Polymer Alkaline Phosphatase with Fast Red.
Completely done on a Vision BioSystem BondMax Immunostainer.

Barry Madigan
Immunohistochemistry
Pathology Queensland
Royal Brisbane Hospital Campus
Australia

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
crochieresteve@aol.com
Sent: Saturday, 6 October 2007 3:59 AM
To: histonet@pathology.swmed.edu
Subject: [Histonet] prostate triple stain

I use the PIN-4 cocktail from Biocare Medical. It works very well.Catalog # 







PPM225DSAA



You will also need the double stain detection kit. I use the automated
version on their stainer, but there's a manual kit available as well.
Call them 800-799-9499

Steve Crochiere HT(ASCP)
Histology Supervisor
New England Pathology Associates
Springfield, MA

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