RE: [Histonet] overinfiltration of brain tissue?

From:"Kemlo Rogerson"

Hi all,
If brain tissue is placed in a vacuum for wax infiltration for too long
a time, what problems can arise?  Can this lead to sectioning problems?
How can the effects be reversed?


If you leave tissue in wax for too long then the heat makes it go hard;
this is especially so with brain tissue which can go so hard as to be
nearly impossible to section. There are some 'recovery' fluids that
include oil of cedar wood but they only mitigate the problem a small

I'm afraid brain is one of those organs that needs 'special' processing.

Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
Giving is more a dictate of the heart than a command of the brain.
--Henry A. Russo 

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