We were (it's been a while) running the Dako PCNA at 1:10000 with a tonsil
control ph6 30/15 using the envision+ detection from Dako.
Ki-67 is requested more for a lot of the studies we deal with.
Bernice

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
AGrobe2555@aol.com
Sent: Wednesday, October 03, 2007 10:02 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re:PCNA

I'm not having much luck either.  I was using colon and esophagus as a  
positive, and getting variable staining (Histochoice fixed tissue).  HIER
made the 
staining worse, not better.  I have some skin that I will be  trying next.  
The ab was used at 1:100 to 1:2000, and 1:400 seemed to work  best.  My 
impression is that PCNA is quirky at best, and I am thinking  about
switching to Ki67 
instead.  

Albert C.  Grobe, PhD
International Heart Institute of Montana Foundation
Tissue  Engineering Lab, Saint Patrick  Hospital





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