Dear Marcy,

I concur,

The primary cause of OCT coming loose from the chuck is the chuck being
pre-cooled in the cryostat. The OCT freezes before it can flow to the bottom
of the grooves and thus it will not hold. Always start with a room temp
chuck and make build-ups one at a time.

Mickie

Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
  & Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
509-954-7134
Web: www.mohshistotemp.com & www.mohslabstaffing.com 
Email: mickie25@netzero.net
 
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MARCELLYN
STONE
Sent: Tuesday, October 09, 2007 7:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CRYOSTAT TROUBLESHOOTING

Hello..

I am looking for some help.  The O.C.T. embedded tissue is falling of the
chuck when we try to section.  Does anyone have any ideas to stop this?  We
have tried new O.C.T., cleaning chucks, advancing slower.  Please help.
Thanks  Marcy

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