Dear Marcy. The chuck is really cold. Take your thumb and warm up the chuck
before you apply the OCT. This solved my similar problem. Good luck. Terri

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MARCELLYN
STONE
Sent: Tuesday, October 09, 2007 7:40 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CRYOSTAT TROUBLESHOOTING

Hello..

I am looking for some help.  The O.C.T. embedded tissue is falling of the
chuck when we try to section.  Does anyone have any ideas to stop this?  We
have tried new O.C.T., cleaning chucks, advancing slower.  Please help.
Thanks  Marcy

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