hey you zany histokids!
All told, Iv'e gotten several Ab's to work on FFPE sections. The
staining is reproducible, clean but it's never super strong. It seems
that any time you have to do enzyme digestion there's a VERY FINE line
between under-digestion and over-digestion of the section and when you
have several dozen to do at once, well, the timing is not always
anyhow, I found a nice paper in Nature from 2006 from Gavin Thurston's
group over at Regeneron. They have these beautiful images of mouse
blood vessels. Of course, being a Nature paper, the details are rather
vague... but, I noticed something interesting...
they used pyronin-y as a counter-stain not hematox. I've personally
never used this or heard of it...
Have any of you used this stain? is it simple to use or are there
"tricks" involved? Can it be used in FFPE sections or just in frozen
As usual, thanks in advance for any help you can give me.
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