[Histonet] re: PCNA

From:Kim Tournear

We always used the Ki67 over the PCNA....docs liked it better and it was always consistent.  Fixative subs can also be issues with HIER and/or enzyme digestion...typically you wouldn't need a harsh HIER or digestion, if any at all, to achieve optimal staining....but not always the case...you just have to be careful of the false (+) and false (-) results....
  If you think about it, most detection kits and antibodies are worked up with 10% NBF for most companies....it's a whole different ball game with fixative subs and, in my world  requires too much and too little time to work up new abs...so we stayed with formalin....
  Just my 2 cents worth....

Kim Tournear, HT (ASCP), QIHC ( ASCP)
  Specialists in Dermatology
  Histology/Mohs Supervisor
  Tucson, AZ

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