We always used the Ki67 over the PCNA....docs liked it better and it was always consistent. Fixative subs can also be issues with HIER and/or enzyme digestion...typically you wouldn't need a harsh HIER or digestion, if any at all, to achieve optimal staining....but not always the case...you just have to be careful of the false (+) and false (-) results....
If you think about it, most detection kits and antibodies are worked up with 10% NBF for most companies....it's a whole different ball game with fixative subs and, in my world requires too much and too little time to work up new abs...so we stayed with formalin....
Just my 2 cents worth....
Kim Tournear, HT (ASCP), QIHC ( ASCP)
Specialists in Dermatology
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