[Histonet] oct falling off the chuck

From:"Amy Farnan"



We find that it works best to warm the cold chuck slightly with your palm before adding OCT. If the chuck is to cold it will not adhere resulting in popping off the chuck.  When you add OCT if it instantly freezes when added warm chuck more before adding. It should go on with out starting to freeze immediately. This will not delay the rapid freeze process once it is put on your cold plate. It will still freeze rapidly for you and will not pop off.

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