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ANyone out there using PCNA?? I having been trying to work up this antibody and am not getting anywhere.? I will be using it in conjunction with?to MIB-1.? Since they are both proliferation markers I would expect them to look very similar, but they do not.? The PCNA seems to show different intensities in the staining where MIB-1 is either positive or negative.? 

I am using breast cancer and colon cancer as my controls.? I have tried using both an HRP and Alk Phos detection.? While the alk phos seems to be a little better than the HRP, it still isn't crisp.? I have the Biocare antibody and titered it out to 1:800 and I am using the Alk Phos Mach-4.

Any suggestions?

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