I was wondering if anyone had been working with or had any knowledge of a couple of products from Diagnostic Biosystems. They are the Histozyme retrieval system and the Intensi/fire DAB enhancement system. I've been using the Histozyme in place of both pepsin and trypsin for 5 minutes at room temperature. The preliminary results have been outstanding. I'ts a big time saver. The enhancement solution has allowed me to use my antibodies at one tenth of the dilutions I normally use. This week I'm in the process of diluting them even further to see what the results are. I'm hoping it will allow me to stop using edta as much as possible and only use citrate retrieval. If anyone has any experience with either of these products please advise me as to what you are doing and how they are working.
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