[Histonet] Washed out nuclear staining???

From:"Greg Dobbin"

Hi Folks,
Mystery to solve.  Some of the surgical cases that came off our stainer
today had very pale washed out looking nuclei. Our H&E control was fine
(ran it twice) so it tells me the problem is either at the source or in
the tissue processor. However, since not all cases are affected, I can't
see how it could be the processor (VIP 5).

So if it is the source, what are we talking about-something other than
formalin in their formalin bottles? Contaminated formalin? Insufficient
fixation time?

Any and all comments will be welcome.
Thanks in advance (again!)

Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!

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