I'm looking for a protocol to label skeletal muscle compartments (T-
tubules, SR...) of frozen sections, using mice antibodies. Mice
muscle was cut and embedded in OCT, frozen using isopentane, and cut
at 8 um. Cover slips were stored at -20C. Expecting details regarding
fixation, permeabilization for better access to all compartments.
Secondary antibodies used are Alexa 488 or 594.
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