I'm not having much luck either.  I was using colon and esophagus as a  
positive, and getting variable staining (Histochoice fixed tissue).  HIER  made the 
staining worse, not better.  I have some skin that I will be  trying next.  
The ab was used at 1:100 to 1:2000, and 1:400 seemed to work  best.  My 
impression is that PCNA is quirky at best, and I am thinking  about switching to Ki67 
instead.  

Albert C.  Grobe, PhD
International Heart Institute of Montana Foundation
Tissue  Engineering Lab, Saint Patrick  Hospital





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