I have been preparing humna skin biopsies for PGP9.5 immunohistochemistry. The
Biopsies are fixed in 2% PLP fixative and cryoprotected in 20%
glycerol/sorennsens buffer. After cutting several specimens on a cryostat they
appear to be insufficinetly fixed. Can tissues that has been cyroprotected be
brought back ro Sorennsens buffered and refixed?
Any suggestions would be appreciated.
Sean Taylor M.D., M.Sc.
Department of Neurology
Kingston General Hospital
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