AW: [Histonet] H&E Staining

From:"Gudrun Lang"

Make the washing steps very short and begin with 96% alcohol, if you use
alcoholic eosin. (15-30 sek. 96%-100%-100%).

Gudrun Lang
Biomed. Analytikerin
Akh Linz
Krankenhausstr. 9
4020 Linz

-----Ursprüngliche Nachricht-----
[] Im Auftrag von William
Gesendet: Mittwoch, 10. Oktober 2007 15:25
Betreff: [Histonet] H&E Staining

 i'm currently trying some H&E staining (post-TUNEL) on paraffin embedded
tissues and keep having the eosin wash out during the dehydration steps
(stepwise: 70% alcohol, 95% alcohol, 100% alcohol)... any ideas on why this
would happen?  am i missing a step in between that allows the stain to
'stick'?  thanks

William Ares
Research Technician
Laboratory of Molecular and Developmental Neuroscience
Weill Medical College of Cornell University
Tel: (212) 746 5056

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