Re: [Histonet] (no subject)
Naira:
Yes, you can. Hydrate the section and do the new protocol. You will not need to repeat the blocking step; start with the new antibody.
René J.
"Margaryan, Naira" wrote:
Hi histoneters:
My question probably very stupid :( I did some immunoperoxide staining
with full protocol (blocking steps, Ab primary and secondary, tertiary,
DAB, hematoxylin counterstained, alcohols, xylin and coverslipped.
My question is: Is it possible to reuse/restain with other Ab this slide
again?
Thanks in advance,
Naira
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