It is always preferable to double stain using DAB for one Ab and DAB+Nickel sulfate for the other Ab; the first will be stained brownish and the second bluish.
  AEC easily fades and is less permanent than DAB besides being similar in colour.
  René J.

Tyler  wrote:
  Morning to All Histonetters
Request from one of the Med Techs I work with. Please can someone
forward or suggest a method for double staining on tissue sections using
DAB and AEC substrate.
Thanks
Marilyn


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