Re: [Histonet] TRAP staining on undecalcified bone
From: | "Tamara Franz-Odendaal" |
Hi
We have done TRAP staining in our lab on undecalcified bone. Refer to
this paper for a great protocol.
Witten, PE., Hansen A., Hall, BK. Journal of Morphology 250: 197-207.
Tamara
Tamara Franz-Odendaal (PhD)
Biology Dept,
Mount Saint Vincent University
166 Bedford Highway
Halifax, NS, B3M 2J6
Canada
Tel: 902 - 457 6140
Fax: 902 - 457 6455
>>> "Tiffany Pitts" 10/31/2006 6:53 PM >>>
Hello all,
I am trying to do TRAP staining on undecalcified mouse bones cut from
Methylmethacrylate embedded blocks. My problem is that I cannot seem to
find
a protocol that works well and/or is on the market any more. Does
anyone
know of a protocol I can use or (even better) a kit? Our in-house
protocol
is light on details and I'm not sure if I'm doing it right.
Thank you
Tiffany Pitts
Department of Urology
University of Washington
Seattle, WA
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