Re: [Histonet] TRAP staining on undecalcified bone

From:"Tamara Franz-Odendaal"


We have done TRAP staining in our lab on undecalcified bone. Refer to
this paper for a great protocol.
Witten, PE., Hansen A., Hall, BK.  Journal of Morphology 250: 197-207.


Tamara Franz-Odendaal (PhD)
Biology Dept, 
Mount Saint Vincent University
166 Bedford Highway
Halifax, NS,  B3M 2J6

Tel: 902 - 457 6140
Fax: 902 - 457 6455

>>> "Tiffany Pitts"  10/31/2006 6:53 PM >>>
Hello all,
I am trying to do TRAP staining on undecalcified mouse bones cut from 
Methylmethacrylate embedded blocks. My problem is that I cannot seem to
a protocol that works well and/or is on the market any more. Does
know of a protocol I can use or (even better) a kit? Our in-house
is light on details and I'm not sure if I'm doing it right.
Thank you

Tiffany Pitts
Department of Urology
University of Washington
Seattle, WA 

Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>