Re: [Histonet] Safranin-O
If such research existed Dr. Churukian would know about it. Alcian Blue
has been the subjected to more research for this application than
Safranin O.
Geoff
George Adams wrote:
>HISTONETTERS:
> I am trying to find out a detailed comparison of Safranin-O
>versus
>Alcian blue stains for glycosaminoglycans and proteoglycans.
>Specifically, does Safranin-O stain sulfated (chondroitin sulfate) or
>only non-sulfated GAGs (hyaluronic acid)? Is there an advantage or
>disadvantage to using Alcian blue for the same glycosaminoglycans? The
>tissue that
>one of the students in our lab will be using is from human umbilical
>cord.
> I received a very nice and quick response from Mr. Charles
>Churukian via Mr. Matthew Frank at the
>the University of Rochester Medical Center which is in bold below.
>
>Mr. Adams,
>
>I was just informed by Charles Churukian that Alcian Blue is the
>correct
>stain to use for an umbilical cord section. Safranin O has been used
>for bone tissue but for this application Alcain Blue is your stain. I
>have no info on comparison studies between the two.
>
>Matthew Frank
>
>
>Looking forward to everyone's responses.
>
>
> George Adams, Ph.D.
>
> Research Associate
>
> University of Texas Southwestern
>Medical Center
>
> Department of Otolaryngology
>
> 5323 Harry Hines Blvd.
>
> Dallas TX 75230-5433
>
> 214-648-0466
>
>
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>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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