Fawn:
  This is how I used to prepare it:
   
  EDTA --- 250g
  Dist. water ---1,750 mL
   
  Add the EDTA to the water in a hot plate with constant agitation (teflon covered magnet). When all the salt is dissolved the solution will have a "milky" appearance. Leave the solution reach room temperature and determine pH (ideally with a pH-meter). The solution will be acid ( wrote:
  Hi everybody,

I am getting ready to decalcify some mouse femurs for immuno staining. 
I know I have to use an EDTA decalcifier, but I do not know how to make 
it. Does anybody have a protocol on this that they would be willing to 
share?

Thanks in advance
Fawn

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